Preparation of radioactive Tay-Sachs ganglioside labeled in the sialic acid moiety.
نویسندگان
چکیده
A procedure is described for the preparation of Tay-Sachs ganglioside specifically labeled in the sialic acid portion of the molecule. Rat brain gangliosides were labeled biosynthetically by the intracranial injection of N-acetyl-(3)H-D-mannosamine. Radioactive gangliosides were isolated and selectively degraded with bacterial neuraminidase and rat liver beta-galactosidase to Tay-Sachs ganglioside-(3)H. Radioactivity in the labeled product was confined to the N-acetyl-neuraminic acid portion of the molecule.
منابع مشابه
The metabolism of Tay-Sachs ganglioside: catabolic studies with lysosomal enzymes from normal and Tay-Sachs brain tissue.
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ورودعنوان ژورنال:
- Journal of lipid research
دوره 11 2 شماره
صفحات -
تاریخ انتشار 1970